Analisis Kemampuan Akumulasi Polyfosfat pada Tiap Fase Pertumbuhan Isolat Bakteri Toleran Uranium
DOI:
https://doi.org/10.33477/bs.v9i1.1320Abstract
Polyphosphate is a straight chain biopolymer consisting of tens to hundreds of phosphate residues that are linked by high energy phosphoanhydride bonds, polyphosphate plays an important role in the bioremediation process of uranium waste, especially in the process of uranium metal precipitation in bacterial cells. Some bacteria are known to have the potential to interact with uranium through redox transformation and bioprecipitation by releasing inorganic phosphate to bind uranium in the environment. Inorganic phosphate results from degradation of polyphosphates that accumulate in cells. This study aims to determine the ability of uranium-tolerant bacteria to accumulate polyphosphates during their growth phase. Measurements were made in 5 growth phases, namely the lag phase, the logarithmic phase, the beginning of the stationary phase, the stationary phase, and the stationary end. Quantitative analysis of polyphosphate accumulations was carried out using the Olsen & Dean method. The test results are known that the uranium-tolerant bacterial isolat is able to accumulate the most optimal polyphosphate in the stationary phase, especially at the 48th hour and the lowest polyP accumulation is found in the initial stationary phase at the 24th hour.References
Clark, J.E., Beegen, H. and Wood, H.J. (1986). Isolation of intact chains of polyphosphate from Propionibacterium shermanii grown on glucose or lactate. J. Bacteriol 168: 1212 - 1219.
Gavigan, J., L.M.Marshall dan A.D.W.Dobson. (1999). Regulation of Polyphosphate Kinase Gene Expression in Acinetobacter baumanni 252. Microbiology 145: 2931-2937.
Groenestijn, J.V., Vlekke, G.J.F.M., Anink, D.M.E., Deinema, M.H. and Zehnder, A.J.B. (1988). Role of Cations in Accumulation and Release of Phosphate by Acinetobacter Strain 210A. App. and Env.Microbiol.54 (12): 2894-2901.
Harold, F.M. and Sylvan, S. (1963). Accumulation of Inorganic Polyphosphate in Aerobacter aerogenes.II. Environmental Control and The Role of Sulfur Compounds. J. Bacterial. 86: 222-231.
Hirota, R., Kuroda, A., Kato, J., and Ohtake, H. (2010). Bacterial Phosphate Metabolism and Its Application to Phosphorus Recovery and Industrial Bioprocesses. Journal of Bioscience and Bioengineering. 109: 423-432.
Hoppert, M. (2003). Microscopic Techniques in Biotechnology. Wiley- VcH Verlag, GmnH & Co, KGaA, Weinheim, Germany.
Jeon, C.O., D.S.Lee, J.M. Park. (2002). Microbial communities in activated sludge performing enhanced biological Phosphorus removal in a sequencing batch reactor. Water Research. 37: 2195-2205.
Kulaev, I.S., Vagabov, V.M. and Kulakovskaya, T.V. (2004). The Biochemistry of Inorganic Polyphosphates;2ed. John Wiley & Sons,Ltd. England.
Kuroda, A., and Ohtake, H. (2000). Molecular Analysis Of Polyphosphate Accumulation In Bacteria. Biochemistry (Moscow) 65 (3) : 304-308.
Mullan, A., J.P.Quinn, and J.W.McGrath. (2002). Enhanced Phosphate Uptake and Polyphosphate accumulation in Burkholderia cepacia grown under Low-pH conditions. Microbial Ecology.44: 69-77.
Muthmainna, H. (2018). KARAKTERISASI FENOTIPIK ISOLAT BAKTERI TOLERAN URANIUM. Biosel (Biology Science and Education): Jurnal Penelitian Sains dan Pendidikan, 7(1), 23-28.
Nishimura, Y., Kairiyama, E., Shimadzu, M., and Iizuka, H. (1981). Characterization of a Radiation-Resistant Acinetobacter. Zeitschrift fur Allgemeine mikrobiologie. 21:125-130.
Olsen, S.R. and Dean, L.A. (1965). Phosphorus Methods of Soil Analysis Part 2. American Society of Agronomy, Inc. USA.
Rao, N.N., Liu, S. and Kornberg, A. (1998). Inorganic Polyphosphate in Escherichia coli: The phosphate Regulon and The Stringent Response. J. Bacteriol. 180(8): 2186
Shiba, T., Tsutsumi, K., Yano, H., Ihara, Y., Kameda, A., Tanaka, K., Takahashi, H., Munekata, M., Rao, N.N., and Kornberg, A. (1997). Proc. Natl Acad. Sci. USA. 94: 11210-11215.
Sidat, M., F.Bux, and H.C.Kasan. (1999). Polyphosphate accumulation by bacteria isolatd from activated sludge. Water SA. 25 no. 2
Swords. W.E., Cannon, B.M. and Benjamin, W.H. (1997). Avirulence f LT2 Strains of Salmonella typhimurium Results from a Defective rpoS Gene. Infection and Immunity. 65(6): 2451-2453.
Tunpiboonsak, S., Mongkolrob, R., Kitudomsub, K., Thanwatanaying, P., Kiettipirodom, Tungboontina, Y., and Tungpradabkul, S. (2010). Role of a Bulkholderia pseudomallei Polyphosphate Kinase In an Oxidative Stress Response, Motilities, and Biofilm Formation. The Jornal of microbiology.48: pp.63-70
Tzeng, C.M. and Kornberg, A. (1998). Polyphosphate Kinase is Highly Conserved In many Bacterial Pathogens. Mol.Microbiol. 29: 381-382.
Vanveen, H.W., Abee, T., Kortstee, G.J.J., Konings, W.N., and Zehnder, A.J.B. (1994). Substrate Specificity of Two Phosphate Transport System of Acinetobacter johnsonii 210A in Relation to Phosphate Speciation in Its Aquatic Environment. The Journal of Biological Chemistry. 269(23): pp 16212-16216.
Wilkinson, J.F., and Duguid, J.P. (1960). The Influences Of Cultural Conditions On Bacterial Cytology. Intern.Rev.Cytol. 9 : 1-76.
Zvyagilskaya, R., Allard, P. and Persson, B.L. (2000). Two systems for Phosphate Uptake in Yarrowia lipolytica Cells Grown at Acidic Conditions. IUBMB Life. 49: 143-147
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms: Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
