KARAKTERISASI FENOTIPIK ISOLAT BAKTERI TOLERAN URANIUM
DOI:
https://doi.org/10.33477/bs.v7i1.389Abstrak
Uranium merupakan unsur radioaktif berbahaya yang bersifat radiotoksik. Meningkatnya penggunaan uranium sebagai bahan bakar pembangkit listrik tenaga nuklir menyebabkan limbah uranium semakin meningkat. Oleh sebab itu diperlukan teknologi remediasi untuk mengolah limbah radioaktif dengan memanfaatkan mikroorganisme. Beberapa bakteri diketahui memiliki potensi untuk berinteraksi dengan uranium melalui transformasi redoks dan biopresipitasi dengan melepaskan fosfat anorganik untuk mengikat uranium dilingkungan. Fosfat anorganik dihasilkan dari degradasi polifosfat yang terakumulasi dalam sel. Penelitian ini bertujuan untuk mengkarakterisasi isolat bakteri potensial pengakumulasi polifosfat yang bersifat toleran terhadap uranium di Indonesia. Karakterisasi dilakukan secara fenotipik yang meliputi identifikasi morfologis, fisiologis, dan biokimiawi. Hasil uji beberapa karakter kunci pada level genus (generic assignment) menunjukkan bahwa seluruh karakter sesuai terhadap genus Acinetobacter. Bentuk sel isolat A671 yang diamati pada fase eksponensial yaitu cocobacil dengan ukuran panjang 1,8 µm dan diameter 1,0 µm, namun pada fase stasioner, bentuk sel berubah menjadi spherical (bulat). Isolat bersifat katalase positif, oksidase negatif, gram negatif, nonmotil, tidak membentuk kapsul dan spora, tidak dapat mengoksidasi 9 jenis sakarida, dan memiliki tingkat toleransi terhadap kadar garam yang tinggi. Kata kunci: Bakteri, Uranium, Karakterisasi FenotipikReferensi
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