Variations in Rhizopus Species That Play a Role in Making Tempeh in the Bekasi Area

Authors

  • Isnain Ardiani Universitas Islam As-Syafiíyah
  • Qurrota Aýun Universitas Islam As-Syafiíyah
  • Karina Sheilla Nazua Universitas Islam As-Syafiíyah

DOI:

https://doi.org/10.33477/bs.v13i1.6816

Abstract

Tempeh is made from fermented soybeans with various types of Rhizopus. The mold used in the making process are known as “ragi”. Ragi plays a key role in converting soybean substrate into healthful nutrition. The tempeh’s ragi may contain more than one species of Rhizopus. This study aims to know a variation of different types of Rhizopus that contributed to the tempeh in Bekasi. The samples are from two different locations are tempeh SMC and JTS. The Methods used in direct planting of PDA media. Macroscopic observations by identifying color, texture and shape of colonies, microscopic slide culture to observe the shape of sporangium, the sporangiophore stalk of columela and sporangiospores. The data is presented in the shape of tables, pictures, and descriptive analysis. Based on the results, found four isolates of the SMC and the two isolate from the JTS. Macroscopic and microscopic in successive ways isolate from tempeh SMC1, SMC2, SMC3, SMC4 has a character similar to R. oryzae, R. stolonifer, R. microsporus and R. oligosporus. As to isolate tempeh JTS1 and JTS2 found similar to R. microsporus and R. oryzae. So, in one tempeh of different locations in the Bekasi, it contains many variations of the Rhizopus.

Keywords: Rhizopus, Tempeh, Variation

Author Biographies

Isnain Ardiani, Universitas Islam As-Syafiíyah

Prodi Biologi FST UIA

Qurrota Aýun, Universitas Islam As-Syafiíyah

Prodi Biologi

Karina Sheilla Nazua, Universitas Islam As-Syafiíyah

Prodi Biologi

References

A’yun, Q., & Janah, L. U. (2022). Isolasi Rhizopus Oligosporus Dan Rhizopus Stolonifer Pada Tiga Tempe Di Kelurahan Jatimakmur, Bekasi. Konservasi Hayati, 18(2), 44–50.

Ari, R., Hastian, & Priambudi Yulistio, A. (2020). Analisis Kualitas Tempe di Pasar Baruga Kendari. Sultra Journal of Agricultural Research, 54–60.

Aryanta, I. W. R. (2020). Manfaat Tempe Untuk Kesehatan. Widya Kesehatan, 2(1), 44–50. https://doi.org/10.32795/widyakesehatan.v2i1.609

Aýun, Q., Suryani, S., & Kurnia, C. (2022). Identidikasi Kapang pada Tempe Bungkus Daun Pisang dan Plastik Asal Pengrajin Tempe Jatiasih, Bekasi. Bioed: Jurnal Pendidikan Biologi, 10(2), 45–51.

Barus, T., Maya, F., & Hartanti, A. T. (2019). Peran Beberapa Galur Rhizopus microsporus yang Berasal dari “laru tradisional” dalam Menentukan Kualitas Tempe. Jurnal Aplikasi Teknologi Pangan, 8(1), 17–22. https://doi.org/10.17728/jatp.3761

Efrida, R., Gultom, Z. A., Zulkarnain, F., & Harahap, E. N. (2022). Sosialisasi Pembuatan Nugget Sehat Berbahan Dasar Tempe Di Desa Petangguhan Kecamatan Galang Kabupaten Deli Serdang. ABDI SABHA : Jurnal Pengabdian Kepada Masyarakat, 3(3), 68–74.

Hartanti, A. T., Rahayu, G., & Hidayat, I. (2015). Rhizopus Species from Fresh Tempeh Collected from Several Regions in Indonesia. HAYATI Journal of Biosciences, 22(3), 136–142. https://doi.org/10.1016/j.hjb.2015.10.004

Ito, M., Ito, T., Aoki, H., Nishioka, K., Shiokawa, T., Tada, H., Takeuchi, Y., Takeyasu, N., Yamamoto, T., & Takashiba, S. (2020). Isolation and identification of the antimicrobial substance included in tempeh using Rhizopus stolonifer NBRC 30816 for fermentation. International Journal of Food Microbiology, 325(April), 108645. https://doi.org/10.1016/j.ijfoodmicro.2020.108645

Maryana, L., Anam, S., & Nugrahani, A. W. (2016). Produksi Protein Sel Tunggal Dari Kultur Rhizopus oryzae Dengan Medium Limbah Cair Tahu Single-Cell Protein Production Through Rhizopus oryzae culture With The Medium Of Tofu Wastewater. Galenika Journal of Pharmacy 132 Journal of Pharmacy, 2(2), 132–137.

Mukhoyaroh, H. (2015). Pengaruh Jenis Kedelai, Waktu Dan Suhu Pemeraman Terhadap Kandungan Protein Tempe Kedelai. Florea : Jurnal Biologi Dan Pembelajarannya, 2(2), 47–51. https://doi.org/10.25273/florea.v2i2.415

Natawijaya, D., Saepudin, A., & Pangesti, D. (2015). Uji Kecepatan Pertumbuhan Jamur Rhizopus stolonifer dan Aspergilus niger yang Diinokulasikan pada Beberapa Jenis Buah Lokal. Jurnal Siliwangi, 1(1), 32–40.

Noviawati, D. A. S., Bintari, N. W. D., & Sudiari, M. (2018). Cemaran Angka Lempeng Total (Alt) Dan Angka Kapang Khamir (Akk) Pada Bolu Kukus Dengan Lama Penyimpanan 3 Hari: Standard Plate Count, Yeast and Mold Plate Count of Traditional Steamed Cake After 3 Days of Storage. Bali Medika Jurnal, 5(2), 257–264.

Nurholipah, N., & Ayun, Q. (2021). Isolasi dan Identifikasi Rhizopus oligosporus dan Rhizopus oryzae pada Tempe Asal Bekasi. Jurnal Teknologi Pangan, 15(1), 98–104. https://doi.org/10.33005/jtp.v15i1.2742

Pitt, J. I., & Hocking, A. D. (2009). Fungi and Food Spolage.Springer. New York.

Sine, Y., & Soetarto, E. S. (2018). Isolasi dan identifikasi kapang Rhizopus pada tempe gude (Cajanus cajan L.). Savana Cendana, 3(4), 67–68. https://doi.org/10.32938/sc.v3i04.487

Surbakti, A. B., Rahayu, S. P., PA, S. M. B., & Ginting, B. R. (2020). Sistem Aplikasi Logika Fuzzy Untuk Penentuan Optimasi Ragi Tempe Pada Proses Fermentasi Tempe Kedelai Menggunakan Metode Fuzzy Mamdani (Studi Kasus : Pengrajin Tempe Kedelai Desa Bulu Cina). Jurnal Ilmiah Simantek, Vol. 4 No.(2), 146–148.

Virgianti, D. P. (2015). Uji Antagonis Jamur Tempe (Rhizopus sp) terhadap Bakteri Patogen Enterik. Majalah Ilmiah Biologi Biosfera, 32(3), 162–168. https://doi.org/10.20884/1.mib.2015.32.3.339

Wahyudi, A. (2018). Pengaruh Variasi Suhu Ruang Inkubasi terhadap Waktu Pertumbuhan Rhizopus Oligosporus Pada Pembuatan Tempe Kedelai. Jurnal Agrium, 3(1), 37–38.

Yarlina, V. P., & Astuti, D. I. (2021). Karakterisasi Kandungan Vitamin B12, Folat dan Isoflavon Tempe Kedelai dengan Isolat Murni Rhizopus oryzae, Rhizopus oligosporus, dan Rhizopus stolonifer sebagai Bahan Pangan Fungsional. Teknologi Pangan : Media Informasi Dan Komunikasi Ilmiah Teknologi Pertanian, 12(1), 92–102. https://doi.org/10.35891/tp.v12i1.2219

Downloads

Published

2024-06-01