Isolasi Dan Uji Aktivitas Bakteri Penghasil Selulase Asal Tanah Kandang Sapi

Albert Sembiring

Abstract


Plant biomass from waste agricultural can be transformed to useful like bioethanol and organic fertilizer in industry and agriculture using bacterial cellulase is one alternative to convert cellulose become organic fertilizer. This research had an objective to obtain potential bacterial cellulase isolated from the soil around of cowshed in Institut Pertanian Bogor. Bacteria were isolated from soil had used serial dilution that spread in 1% Carboxy Methyl Cellulose Medium (CMC 1%) and it's incubated at 37oC for 48 hours. Qualitative test for bacteria producing cellulose was carried out by picking the selected colony in the centered of CMC medium with adding congo red 1%. Based on the results were obtained three potential isolates could produce cellulose that were KS 0.1, KS 0.7 and KS 9.1. The highest cellulolytic index is 1.33 that produced by KS 0.1. The test quantitative activity of KS 0.1 using spectrophotometry method showed that the highest of enzyme activity at the first day and lowest eighth day during incubation period.
Key Words: bacteria, cellulose, soil around of cowshed

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Abalos, J.M.F., Arribas, A.R., Garda, A.L., Santamaria, R.I. (1997). Effect of carbon source on the expression of celA1, a cellulase-encoding from Stretomyces halstedii JM8. FEMS Microbiol Letters 153:97-103.

Alam, M.Z., Manchulur, M.A., dan Anwar, M.N. (2004). Isolation Purification, Cellulolytic Characterization of by Enzyme Produced the Isolate Streptomyces omiyaensis. Pakistan Journal Biology Science. 7(10):1647-1653.

Enari, T.M., dan Paavola-Niku, M.L. (1987). Enzymatic of cellulose: Is the current of the mechanisms of hydrolysis valid CRC Crit. Rev. Biotechnology. 5:67-87.

Gerhartz, W. (1990). Enzymes in Industry: Production and Applications. VCH Verlagsgesellschaft mbH, D. 6940 Weinheim. 81-82.

Goto, M., Furukawa, K., Hayashida, S. (1992). An avicel-affinity site in an avicel-digesting exocellulase from Trichoderma viride mutant. Bioscience Biotechnology. 56(10): 1523-1528

Howard, R.L., Abotsi, E., J. van Rensburg E.L., and Howard, S. (2003). Lignocellulose Biotechnology: Issue of Bioconversion and Enzyme Production. African Journal of Biotech. 2(12):602-619.

Indrawati, I. dan S. Djajasupena. (2005). Isolasi Jamur dari Seresah dan Uji Keefektifannya dalam Penguraian Selulosa. Jurnal Ilmiah Biologi. 4(2):18 – 21.

Khairiah E, Khotimah S, Mulyadi A. Karakterisasi dan Kepadatan Bakteri Pendegradasi Selulosa pada Tanah Gambut di Desa Parit Banjar Kabupaten Pontianak. Jurnal Protobiont. 2(2):87-92.

Li, X., Gao, P. (1997). Isolation and Partial Properties of Cellulose-Decomposting strain of Cytophaga sp. LX-7 from soil. Journal of Applied Microbiology. 82:73-80.

Martina, A., Yuli, N., Sutisna, M. 2002. Optimasi Beberapa Faktor Fisik Terhadap Laju Degradasi Selulosa Kayu Albasia (Paraserianthes falcataria (L.) Nielsen dan Karboksimetilselulosa (CMC) secara Enzimatik oleh Jamur Jurnal Natur Indonesia. 4(2):156-163.

Meryandini, A., Widosari, W., Maranatha, B., Sunarti, T.C., Rachmania, N., Satria, H. (2009). Isolasi bakteri selulolitik dan karakterisasi enzimnya. Makara Sains. 13(1): 33-38.

Miller, G.L. (1959). Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry. 31(3): 426-428.

Mtui, Y.S. (2009). Recent Advances in Pretreatment of Lignocellulosic Wastes and Production of Value Added Products. African Journal of Biotechnology.8(8):1398-1415.

Purwadaria, T., Marbun, P.A., Sinurat, A.P., Ketaren, P.P. (2003). Perbandingan Aktivitas Enzim Selulase dari Bakteri dan Kapang Hasil Isolasi dari Rayap. Jurnal Ilmu Ternak dan Veteriner. 8(4):213-219.

Saha, B.C. (2004). Lignocellulose Biodegradation and Application in Biotechnology. US Government Work. American Chemical Society. 2-14.

Sari, U.M., Nurmiati, Agustien, A. (2012). Penapisan dan Karakterisasi Bakteri Selulolitik Termofilik Sumber Air Panas Sungai Medang, Kerinci, Jambi. Jurnal Biologi Universitas Andalas. 1(2):166-171.

Sazci, A., Radford, A., Erenler, K. (1986). Detection of cellulolytic fungi by using Congo red as an indicator:a comparative study with the dinitrosalicyclic acid reagent method. Journal of Applied Bacteriology. 61. 559-562.

Yogyaswari, S.A., Rukmi, M.G.I., Ruharjo, B. (2016). Eksplorasi Bakteri Selulolitik dari Cairan Rumen Sapi Peranakan Fries Holland (PFH) dan Limousine Peranakan Ongole (LIMPO). Jurnal Biologi. 5(4):70-80.




DOI: http://dx.doi.org/10.33477/bs.v8i1.843

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